Abstract: The global epidemic of Hepatitis B virus (HBV) is one of the most serious public health problems in the world and Blood transfusion is one of the most important transmission pathways of HBV. The present study was conducted to determine the prevalence of Hepatitis B in blood donor in Iran. This study was constructed based on the computerized literature database. English and non-English Articles were searched in PubMed database, ISI Web of Science, Iranmedex, Magiran, Google Scholar, SID, Scientific Journal of Iran Blood Transfusion Organization (SJIBTO) and Directory of Open Access Journals (DOAJ) from 1996 to 2008. Infection rates and 95% CI calculated by random affect model. Statistical analyses were performed using STATA 10.0, R Ver. 2.3 and ArcGIS 3.2a was used for map construction. Thirty papers from 83 studies involving 23671296 patients met our inclusion criteria. The prevalence of HBV infection in blood donors was 0.7% (CI 95: 0.5-0.8%). The highest rate of prevalence of hepatitis B in blood donors were found in Gilan,-Mazandaran,-Golestan and Semnan provinces with 1.1% (CI 95:0.6-1.6%). Prevalence of hepatitis B in blood donors from 1996-2008 is decreased, therefore national vaccination of hepatitis B after 1993, awareness programs and screening of bloods transfusion has led this fact.