Abstract: Dwindling supplies of petroleum and growing environmental concerns over its use has led to increasing interest in developing biomass as a feedstock for liquid fuels. In particular, bioethanol produced from biomass represents a promising alternative fuel or gasoline extender. Bioethanol is used in vehicles either as a sole fuel or blended with gasoline. As an oxygenated compound, ethanol provides additional oxygen in combustion and hence obtains better combustion efficiency. The main environmental advantages of fuel ethanol are its sustainability in using a renewable resource as a feedstock, thus promoting independence of fossil fuel and maintaining the level of greenhouse gas. Bioethanol can be produced from carbohydrate containing substrates by the process of fermentation. Many microorganisms like bacteria, fungi and yeast involved in bioethanol production. Present study deals with bioethanol production from rotten fruits with inoculation of bacteria isolated from respective fruits. Rotten fruits serve as potential feedstock for bioethanol production due to high sugar content and cost effective substrate. Results indicate that among 5 fruits rotten sapota (Manilkara zapota) produced highest amount of bioethanol 9.40% on 5th day of incubation. Bioethanol can solve the problem of pollution and considered as fuel for future.