Abstract: Rosa damascena has been used as a traditional remedy for constipation and many other digestive disorders. In the current research, the effects of the aqueous fraction of R. damascena extract on the histaminergic and muscarinic receptors of the Guinea pig ileum and rabbit jejunum were investigated. First, the effects of different concentrations of the aqueous fraction on the contractions of ileum were examined. Then, the effects of the same concentrations of the aqueous fraction on ileal contractions, in the presence of chlorpheniramine and atropine, were recorded and measured. Furthermore, the effects of the aqueous fraction and acetylcholine on the contractions of jejunum were separately recorded, both before and after antagonizing the cholinergic receptors by atropine. To maintain a constant standard, the increase in contractile response of Guinea pig ileum to 0.1 μg mL-1 of histamine (equal to 10 cm increase in the amplitude of basal contractions) and increase in contractile response of rabbit jejunum to 1 μg mL-1 of acetylcholine (equal to 8 cm increase in the amplitude of basal contractions) were considered as 100% response. Aqueous fraction of Rosa damascena significantly increased the amplitude of basal contractions of rabbit jejunum and Guinea pig ileum. After antagonizing the histaminergic receptors, there was a considerable decrease in the contractile response of ileum to aqueous fraction. Also, contractile response of ileum and jejunum to aqueous fraction was decreased after antagonizing the cholinergic receptors. The reduction in contractile response to aqueous fraction following blockade of cholinergic receptors by atropine was more considerable in rabbit jejunum. These findings suggest that the stimulatory effect of the aqueous fraction of R. damascena on the intestinal contractions can be due to its excitatory effect on histaminergic and cholinergic receptors of ileum and jejunum.