Abstract: Black Grams are capable of fixing atmospheric nitrogen through Rhizobium species living in its root nodules. To evaluate the effect of different Rhizobium strains on yield of Black Gram, a field experiment was conducted in the Rajshahi University field of Bangladesh. Three Rhizobium strains (RLc 107, RCa 220 and RVm 307), four different types of adhesives (Sucrose, Peptone, Molasses and Glycerol) and two Black Gram varieties (BARI MASH-1 and BINA MASH-1) were used as an experimental material. Data were recorded on number of pods per plant, number of seeds per plant, 100-seed weight and seed yield per plant. The yield of Black gram was significantly affected by Rhizobium inoculation and the better result for all the characters were obtained in BINA MASH-1 when seeds were inoculated with RVm 307 using the adhesive peptone.