Abstract: Recombination plays a key role in the evolution of Begomovirus and may be contributing to the emergence of new species. With the development of computational recombination detection tools and an increasing number of available genome sequences, many studies have reported evidence of recombination. Begomovirus associated symptoms were observed in Jasminum sambac and Millingtonia hortensis plants growing in crop fields of Lakshmangarh, Rajasthan (India). Amplification of a PCR product was found up to the expected size (~550 bp). The PCR product was cloned and partially sequenced and it was utilized for in silico characterization. The in silico analysis suggested that interspecific recombination has resulted in significant diversity among Begomovirus.