Abstract: Methanol extracts of leaves of Anogeissus leiocarpus were screened for antimicrobial activity against Salmonella typhi, Escherichia coli, Staphylococcus aureus and Shigella sp. at the Biological Science Laboratory of the University of Agriculture, Makurdi. The disc diffusion method was used to assay for the antimicrobial property on the test isolates. The extracts at different concentrations inhibited the growth of S. typhi, E. coli and Shigella sp. The minimum inhibitory concentration of the extraction for S. typhi, E. coli and Shigella sp. were 49.73, 30.62 and 33.17 mg mL-1, respectively. The antibacterial activity may likely be due to the presence of some active compounds present in the plant extract. Further, preliminary phytochemical screening of the leaves extract of this plant is suggested.