Abstract: Urinary tract infection is a very common disease that can affect anyone at any age where the infection rate is higher in woman than man. This study aimed to ascertain the current situation of Urinary Tract Infections (UTIs) and drug resistance determination caused by Escherichia coli in different age groups of patients. This prospective study determined the antibiotic susceptibility of 131 isolates of Escherichia coli from the urine culture of 665 patients. Urine samples were aseptically collected in sterile containers and then cultured on Nutrient media. Susceptibility to 9 antimicrobial agents was tested by the disc diffusion technique. Data analysis was performed by employing SPSS software, Version 10.0 and group’s t-test was done as a test of significance. Among 131 E. coli confirmed patients 69 were females and 31% were males. It state that female are more vulnerable to UTI than male (p<0.001). The onset of UTIs in female was high at the age between 21-30 years (28.90%). The occurrence of the disease was high in March-October. Studies revealed that the most resistance rates for E. coli detected from urine culture were Amoxicillin, Doxycycline, Cephalexin, Cephradine, Cotrimoxazole, Cefixime, Ceftroxone and Ciprofloxacin except, Cefuraoxime which was significantly sensitive (p<0.001). This study was evaluated for the prevalence of E. coli in UTI especially to the women and the antibiotic resistance pattern of E. coli.