Abstract: As the prompt and accurate diagnosis is important for undertaking an effective control measure, the present study was undertaken to evaluate four serological tests for diagnosis of Brucella infection in goats and cattle in some selected areas of Bangladesh. Sera were collected from goats (n = 108) and cattle (n = 60). All sera were screened for Brucella specific antibody response by the rose bengal plate test (RBT), slow agglutination test (SAT), indirect enzyme-linked immunosorbent assay (i-ELISA) and competitive ELISA (c-ELISA). Isolation of Brucella organisms was performed by culturing of blood samples onto bacteriological media and identification of the organism was conducted by Gram’s staining and biochemical tests. The sensitivity and specificity of the serological tests were estimated as follows: in goats, RBT: 100 and 93.40%, SAT: 100 and 96.22%, i-ELISA: 66.67 and 92.38%, c-ELISA: 100%, respectively and in cattle, RBT: 100 and 94.92%, SAT: 100 and 96.61%, i-ELISA: 50 and 93.10%, c-ELISA: 100%, respectively. RBT was found as a suitable screening test and c-ELISA could be used as a confirmatory test for the diagnosis of Brucella infection.