Abstract: This study was carried out to investigate the nutrients composition of Nigella sativa seed and its minerals content, cultivated in three different regions in Yemen, namely Marib, Sadah and Taiz. Proximate analysis, Total Dietary Fiber (TDF), Insoluble Dietary Fiber (IDF) and Soluble Dietary Fiber (SDF) were determined using the Association of Official Analytical Chemists methods. Minerals content of N. sativa seeds was determined by the Atomic Absorption Spectrophotometry (AAS). The proximate analysis of N. sativa seeds showed that moisture content was 6.8±0.3, 4.6±0.1 and 7.2±0.14% for Marib, Sadah and Taiz samples, respectively. The seeds showed to have high content of TDF which was 36.88±1.44, 26.50±1.05, 30.40±1.06%, IDF 27.10±0.55, 20.56±1.16, 22.40±1.40% and SDF 8.90±1.17, 6.50±0.60, 8.13±0.71% for Marib, Sadah and Taiz samples, respectively. Minerals content analysis of N. sativa seeds showed the seeds to be a rich source of calcium, magnesium, potassium, phosphorus and iron. Present results revealed that N. sativa seeds contain several nutrients with potential value.