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American Journal of Biochemistry and Molecular Biology

Year: 2014 | Volume: 4 | Issue: 3 | Page No.: 136-142
DOI: 10.3923/ajbmb.2014.136.142
Wild Plants as Sources of the Permanency of Viruses Infecting Cultivated Plants: Case of Cassava Begomoviruses in Togo
Adjata Kossikouma Djodji , Agbelessessi Yao Woegan, Assion Setu Mivedor, Emmanulle Muller, Michel Peterschmitt, Komla Sanda and Yawovi Mawuena Dieudonne Gumedzoe

Abstract: A study was carried out on some wild plants in order to understand the permanency of Cassava Begomoviruses diseases in Togo. Leaves were collected in cassava fields from cassava and some wild plants (Albizia zygia (DC) J.F. Mabr, Senna hirsuta (L.) H.S. Irvin and Barneby, S. obtusifolia (L.) H.S. Irvin and Barneby, S. occidentalis (L.) Link., Manihot glaziovii Müll. Arg., Pupalia lappacea (L.) Juss., Strophanthus hispidus DC) that exhibiting leaf curling, distorting and chlorotic lesions near the veins and stunting which were similar to the symptoms of Begomovirus infection and were analyzed by PCR assays with degenerate primers. Symptomatic leaf tissues from some of these infected plants were ground in 0.1 M phosphate buffer (1:1, w/v pH7.0) and squeezed through double layered muslin cloth and the filtrate was mechanically inoculated to several indicator species. Consistent amplification of DNA fragment was obtained (770 bp fragments) from symptomatic plants. The PCR analysis revealed the association of Cassava mosaic begomovirus in Albizia zygia, Senna obtusifolia, Manihot glaziovii, Pupalia lappacea, Strophanthus hispidus. The presence of the virus was confirmed in host inoculated plants. The presence of the virus was confirmed in inoculated host plants analysed by PCR.

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How to cite this article
Adjata Kossikouma Djodji, Agbelessessi Yao Woegan, Assion Setu Mivedor, Emmanulle Muller, Michel Peterschmitt, Komla Sanda and Yawovi Mawuena Dieudonne Gumedzoe, 2014. Wild Plants as Sources of the Permanency of Viruses Infecting Cultivated Plants: Case of Cassava Begomoviruses in Togo. American Journal of Biochemistry and Molecular Biology, 4: 136-142.

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