Abstract: Knowing the appropriate applications of various analytical techniques used in biotechnology, makes the experiment more easier and let the researcher obtain a better result which also reduces the time period of experiment by eliminating the trial and error methods. Along with the knowledge of application of the analytical technique, the researcher also should have the knowledge of the sampling techniques for the analytical instruments as it is one of the deciding factors to choose a specific technique for the analysis. At the same time, the results obtained by using the analytical equipments are relatively authentic and accurate. With the development of biotechnology, the usage of analytical equipments has increased by many folds. The areas of biotechnology such as nanotechnology, cancer biology, genetic engineering and many more are dependent on the analytical equipments for most of their experimental analysis. Analytical equipments find its application not only in the research works but also at the industry level. Various food and pharmaceutical industries rely upon the analytical techniques like FT-IR and Raman for the quality analysis of the products.