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Asian Journal of Biotechnology

Year: 2012 | Volume: 4 | Issue: 2 | Page No.: 92-99
DOI: 10.3923/ajbkr.2012.92.99
Plant Regeneration Through Direct Shoot Formation From Sago Palm (Metroxylon sagu Rottb.) Leaf Explants
A.U. Novero and F. Jamiri

Abstract: Sago palm (Metroxylon sagu) is an emerging important food plant for marginal lands. The palm reproduces in the wild slowly by means of suckers. Thus, in vitro culture methods must be employed to aid its multiplication. In this study, varying concentrations of auxin and cytokinin were evaluated to establish appropriate media for shoot regeneration of sago palm (Metroxylon sagu Rottb.) leaf explants. Shoots that regenerated from modified Murashige and Skoog (MS) medium containing 10 mg L-1 2,4 D and 0.2 mg L-1 kinetin with 0.3% activated charcoal, 3% sucrose and 2 g L-1 gelrite at pH 5.7 showed the greatest weight increases. However, shoot explants inoculated in media adjusted to pH 6.8 showed faster morphological changes.

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How to cite this article
A.U. Novero and F. Jamiri, 2012. Plant Regeneration Through Direct Shoot Formation From Sago Palm (Metroxylon sagu Rottb.) Leaf Explants. Asian Journal of Biotechnology, 4: 92-99.

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