Abstract: The objective of the present study was to determine the effect of genotype and culture media in anther culture of boro rice hybrids. Anthers from three f1 hybrids of boroxhigh yielding indica rice varieties GautamxBPT 5204, Krishna HansaxNDR 359 and IR64xNDR 359 were cultured on modified SK media and modified N6 media. Modified SK media was found to be more suitable for callus formation in all the f1 hybrids. The cross GautamxBPT 5204 showed maximum callus induction frequency followed by Krishna HansaxNDR 359. Only the cross of Krishna HansaxNDR 359 was responsive to green plant regeneration while IR64xNDR359 and GautamxBPT 5204 resulted in only albino plants. Anther culture response is influenced by the genotype and media composition in indica rice hybrids.