Abstract: Objective: To investigate the renoprotective potentials of Vernonia calvoana extract (VCE) in acetaminophen treated Wistar rats. Materials and Methods: Thirty-five male Wistar rats (100-150 g) were randomly assigned to 5 groups of 7 rats each. Group 1 (normal control) received normal saline, group 2 received normal saline after oral administration of 2 g kg–1 b.wt. of acetaminophen, groups 3 and 4 were administered 200 and 400 mg kg–1 b.wt. of VCE, respectively after pre-treatment with acetaminophen. Group 5 was administered 100 mg kg–1 b.wt., of Vitamin E. All treatments were given orally and study lasted for 21 days thereafter blood was collected for measurement of kidney function biomarkers. One-way analysis of variance was used for analysis followed by post hoc multiple comparisons and SPSS. Results: The results showed increased serum sodium concentration in group 3 and 4 administered VCE when compared with the acetaminophen treated control, unlike the Vit E supplemented group which showed a decrease (p<0.001). Conversely, serum potassium and urea concentrations were decreased in the VCE treated groups compared to the acetaminophen treated control (p<0.001). Further, serum creatinine, a notable marker of kidney function was decreased in VCE treated groups in tandem Vit. E supplemented group when compared to acetaminophen-treated control. Conclusion: It was concluded that extracts of Vernonia calvoana leaves potently ameliorated imbalance in kidney function biomarkers induced by acetaminophen, hence may possesses renoprotective potentials.