Abstract: Background and Objective: Nauclea latifolia root-bark extracts are used traditionally to treat various diseases. The study investigated phytochemical compositions and effects of methanol extract of Nauclea latifolia root-bark on liver and kidney function parameters of alloxan induced diabetic male wistar rats to understand possible effect it could have on those who use the extract to manage diabetes. Materials and Methods: The acute toxicity study was carried out using 18 male mice while 30 male Wistar albino rats were used to study the effects of the extract on the liver and kidney function parameters using standard analytical methods. The rats were divided into six groups of five rats each with group 1 as normal control, group 2 as the negative that was alloxan induced but not treated, group 3 rats were alloxan induced but treated with 2.5 mg kg–1 body weight glibenclamide . Group 4-6 rats were alloxan induced but treated with 100, 200 and 400 mg kg–1 body weight of the methanol extract respectively for 14 days. The data obtained were statistically analyzed using one way analysis of variance at 95% confidence level. Results: Phytochemical analysis of the extract showed high concentrations of pharmacological active phytochemicals like flavonoids, saponins, proteins, carbohydrate and alkaloids. The acute toxicity study showed that the extract was relatively safe as no death or adverse reactions were observed after extract was administered to the mice. The induction of diabetic condition in rats with alloxan resulted in significant (p<0.05) increase in activities of liver marker enzymes (AST, ALT and ALP) concentrations of total bilirubin, urea, creatinine and serum electrolytes. Treatments with glibenclamide and the methanol extract, respectively showed significant (p<0.05) decreases in the activities of these liver marker enzymes and concentrations of total bilirubin, urea, creatinine and serum electrolytes when compared to the negative control. The effects of the methanol extract were inversely proportional to the dose of the extract administered. Conclusion: The findings of this study suggest that the methanol extract possesses hepatoprotective and pharmacological activities that could promote liver and kidney function and thus, could protect these organs from both endogenous and exogenous stressors.