Abstract: Background and Objective: Gel of Musa acuminata stem from South Borneo had shown to accelerate wound healing by increasing the number of neovasculars on the 5th day. The study aimed to determine the effect of gel of Musa acuminata stem extract (EBPM) towards increased expression of HIF-1α, Hsp90α and VEGF in healing traumatic ulcer. Materials and Methods: This research was a true experimental with 40 rats of Rattus norvegicus of male Wistar strain as the traumatic ulcer models. The biopsy was taken from the ulcer in the left buccal oral mucous. The study was divided into the negative control group (K) given gel, treatment group 1 (P1) given gel of 25% EBPM, treatment group 2 (P2) given gel of 37.5% EBPM and treatment group 3 (P3) given gel of 50% EBPM. On the 3rd and the 5th days, the models were decapitated. They were next biopsied in order to make preparations and to conduct immunohistochemistry test. Results: The results proved that the EBPM gel was effective when administered at a concentration of 37.5% in the 5th day because it significantly increased the expressions of HIF-1α, Hsp90α and VEGF. Conclusion: It can be concluded that gel of Musa acuminata stem extract of 37.5% could increase neovascular number through increasing the expression of HIF1α, HSp90α and VEGF to accelerate oral ulcer healing, including traumatic ulcer. It has indicated that gel of Musa acuminata stem extract of 37.5% concentration can be recommended as a natural ingredient of topical drug to accelerate ulcer healing in oral mucous.