Abstract: The purpose of the study was to investigate the anti-depressant like effect of ethanolic extract of H. hookerianum (EEHh 200 and 400 mg kg–1 p.o.,) and its glycosidic flavonoid enriched extract of ethanolic extract of H. hookerianum (GFHh 100 mg kg–1 p.o.,) in reserpine induced (2 mg kg–1 i.p.,) Swiss albino mice. The flavonoidal constiteuents in EEHh and GFHh was confirmed by HPLC. The behavioral analysis of forced swimming test, tail suspension test, locomotor activity in mice has been conducted. Brain superoxide dismutase, catalase, glutathione and lipid peroxidation were estimated biochemically. Brain monoamine oxidase (A and B) activity has been carried out by double beam spectrophotometer. All the values in this study indicated that the GFHh posses well specified antidepressant activity than EEHh due to higher concentration of flavonoids as confirmed by HPLC.