Abstract: Artemisia abyssinica, belongs to the family Asteraceae, is an herb commonly used in folk medicine. Antibacterial and antioxidant activities of six organic solvent extracts (n-hexane, dichloromethane [CH2Cl2, 100%], dichloromethane:methanol [CH2Cl2:MeOH, 1:1], methanol [MeOH, 100%], methanol:water [MeOH:H2O, 8.5:1.5] and water [H2O, 100%]) of the aerial parts of A. abyssinica extracts were investigated. Antibacterial activity was evaluated using the agar diffusion assay against Gram-negative Escherichia coli and Pseudomonas aeruginosa and Gram-positive Staphylococcus aureus and Bacillus megaterium bacterial strains. The minimum inhibitory concentration of the Artemisia abyssinica extracts was tested using a two-fold dilution method at concentrations ranging from 32-512 μg mL-1. Antioxidant activities were measured using the 1,1-diphenyl-2-picrylhydrazyl and superoxide dismutase-like activity methods. Among the tested extracts, n-hexane and water extracts exhibited strong antibacterial activity. These extracts also exhibited good antioxidant activity with a 50% inhibition concentration. The extracts were also screened for their degradation effects of high molecular weight biomolecules, such as DNA and bovine serum protein.