Abstract: Liver fluke infection (fascioliasis) is now neglected tropical disease caused by Fasciola hepatica and F. gigantica. Snails of family Lymnaeidae are the intermediate host of F. gigantica. Snail control is considered as one of the best method of choice to eliminate fascioliasis. Bait formulation containing molluscicides would be an effective tool to snail control. There was a significant (p<0.05) reduction in protein, amino acids, DNA, RNA and AChE activity in ovotestis/nervous tissue of snail Lymnaea acuminata. Feeding of 96 h exposure period of bait containing molluscicides Piperine+Serine+Agar (40% of 24 h LC50) caused maximum reduction in protein (75.27% of control) in month of August, amino acids (78.37% of control) in month of March, DNA (72.47% of control) in month of May and RNA (75.03% of control) in month of October in ovotestis of L. acuminata. There was a significant (p<0.05) inhibition in AChE activity with the feeding of bait containing plant derived molluscicides Piperine+Serine+Agar (40% of 24 h LC50) of 96 h exposure period. Maximum inhibition in AChE activity (46.42% of control) in month of October was noted in nervous tissue of L. acuminata. Notably, the bait containing molluscicides is new technique effectively approaching target snails.