Abstract: Wedelia chinensis is a deciduous tree traditionally considered as cardio tonic but has not been proven scientifically. Therefore, the present study aimed to evaluate the cardio-protective effect of W.chinensis on isoproterenol induced myocardial infarction in rat. The experimental albino rats were divided into four groups. The hydro-ethanolic extract of W.chinensis was orally administered to group 3 and 4 rats for 28 days. Isoproterenol (20 mg/100 g) subcutaneously injected twice at an interval of 24 h to the group 3 rats on 29 and 30th day. At the end, rats were sacrificed and collected serum and heart samples for biochemical and histopathological studies. Significant (p<0.05) results were observed on protein, urea, creatinine, serum lipid profile, cardiac marker enzymes and antioxidants in group 3 rats nearer to normal. Histopathological observation clearly showed the cardioprotective action of the study plant. Further studies will identify the bioactive molecule responsible for the cardioprotective activity.