Abstract: The present study was designed to evaluate the antimicrobial, radical scavenging and chemical composition of aerial parts of Cassia auriculata. Three hundred grams of powdered material of C. auriculata extracted with methanol (CAMEt), C-18 silica gel based column chromatography was used to purify CAMEt using n-hexane, ethyl acetate and methanol fractions. Antimicrobial, DPPH radical scavenging effect of all the three fractions was determined and active fraction was characterized by GC-MS. The yield and colour of each fractions collected from column chromatography viz., fraction 1-3 (n-hexane: yellow) was 2.45 mg, (ethyl acetate: light orange) 1.78 mg and (methanol: light green) 5.25 mg, respectively. Fraction 3 recorded maximum zone of inhibition against Pseudomonas aeruginosa, Aspergillus niger and Penicillium sp. by fraction 3 antifungal and radical scavenging activity than compared to fractions. GC-MS results indicated 21 chemical constituents included alkanes, alcohol, esters and hydrocarbons. The major peak represented 1, 2-Benzenedicarboxylic acid and Di-(2-ethylhexyl) phthalate at 11.42% referred to NIST library. Further studies will be found in the mechanism of potential methanol fraction.