Abstract: Botanical insecticides have been recommended by most of the Biointensive Integrated Pest Management (BIPM) Practitioners throughout the world. However, the target organs for such botanicals are imperative. Here, the toxicological effects of Tephrosia purpurea (Linn.) (Fabaceae) and Acalypha indica (Linn.) (Euphorbiaceae) crude extract on the mortality, reproductive organs macromolecules, mineral level in alimentary canal and detoxication enzyme level in the fat body and intestine of Dysdercus cingulatus (Fab.) (Pyrrhocoridae) was studied. Crude extract of A. indica caused more mortality than T. purpurea. Cotton leaves and seed were altered the impact of these botanicals pesticidal property. For instance, the LC50 value was higher when the pest was fed with aqueous extract treated cotton seeds than on cotton leaves. Detoxication enzymes like Aspartate Aminotransferase (AAT) and Alanine Aminotransferase (ALT) level was high in intestine than the fat body whether the insect fed with cotton leaves or cotton seeds. AAT level was enhanced by the T. purpurea extract than A. indica extract. The Na+ level high in cotton seed fed pest whereas K+ level and Ca++ level were high in cotton leaves fed animals. A. indica sprayed cotton seed induce the Na+ and K+ level in the alimentary canal. Ca++ level decreased by A. indica and T. purpurea extracts sprayed cotton seed and leaves fed pest. Treatment of T. purpurea and A. indica altered both total glucose and protein level of D. cingulatus ovary and tests. It seems that the detrimental effect of the botanical crude extract can be due to both the modulating nature of the extracts and the biochemical alteration in the respective system, alimentary canal and fat body of the D. cingulatus can be a potent candidate in integrated pest management programmes for controlling such pests.