Abstract: This search aims to find a correlation between adhesion molecules and fatty liver patients. Raised serum levels of endothelial-leukocyte adhesion molecule-1 (E-selectin), intercellular adhesion molecule-1 (ICAM-1) and circulating vascular cell adhesion molecule-1 (VCAM-1) have been observed mainly in patients with infections, inflammatory and fatty liver disease. Non-Alcoholic Fatty Liver Disease (NAFLD) was diagnosed by persistently elevated ALT and ultrasonographic bright liver with no other liver disease. A study was carried out to analyze whether fatty liver patients with increasing cholesterol and triglycerides is related to the serum levels of E-selectin, ICAM-1 and VCAM-1. Ninety serum samples from NAFLD patients were classified to four groups. The results showed that ALT and AST were significantly higher in fatty liver patients with high TG and cholesterol (group 1), compared to controls (92.16±31.197 vs. 33.56±8.322, p<0.001) for AST and (76.8±28.872 vs. 29.32±6.479, p<0.001) for ALT. While no significant difference was observed between ALT levels of fatty liver (with normal TG and cholesterol) and controls (32.36±7.745 vs. 29.32±6.479, p = 0.05). Positive E-selectin samples were found in 88% of patients in group (1), 90% in group (2), 100% in group (3) and 32% in group (4). These values were significantly higher than those in healthy individuals (p<0.0001). High ICAM-1 level was found in 80% in-group (1), 75% in-group (2), 100% in group (3) and 80% in group (4). Level of ICAM-1 in group (1) was extremely significant compared to group (2) (465.44±56.501 vs. 407.48±32.686 p<0.0001 ES). The values of VCAM-1 in group (1) were significantly higher than those in healthy individuals (p<0.0001) and positive VCAM-1 samples were found in 72% in group (1), 35% in group (2), 40% in group (3) and 0% in group (4). In conclusion, there was a significant increase in circulating levels of ICAM-1, VCAM-1 and E-selectin in NAFLD compared to healthy control subjects and it may be used to comprehensively using the ability of circulating VCAM-1, E-selectin and ICAM-1 to predict fatty liver disease and evaluated the relationship between circulating adhesion molecules and fatty liver.