Abstract: The dental pulp tissue is essential in dentine development. The existence of Dental Pulp Stem Cells (DPSCs), i.e., osteoblast and odontoblast are to assist in dentine repair and tooth regeneration. The existence of osteoblast that secreted bone matrix directly from pulp tissue has not been reported. The purpose of this study is to determine the existence of odontoblast and osteoblast cells excavated directly from pulp tissues by using molecular markers. The isolated RNA expressing two gene markers, i.e., dentin sialophosphoprotein and osteocalcin which were secreted by odontoblast and osteoblast cells, respectively. The expression of dentin sialophosphoprotein and osteocalcin demonstrated that both odontoblast and osteoblast cells exist in adult human pulp tissues.