Abstract: In the present study mice were supplemented with 2% of both L-arginine and L-ornithine in drinking water for four weeks. L-arginine and L-ornithine intake elevate polyamine levels in serum of female Swiss albino mice. The effect of selenium (Se) administration (as sodium selenite: 0.5 or 1 mg kg-1 body weight) or/and α- difluromethylornthine (DFMO: 2 mg kg-1 body weight) on the elevated polyamine levels was studied. The elevated polyamine levels were decreased significantly by administration of low and high doses of Se with DFMO. Glucose concentration in the serum increased significantly with high polyamine level of groups and reduced back around the normal values by Se and DFMO treatment. The concentrations of triglycerides and cholesterol are not effected by the elevated levels of polyamines in the serum. These results suggest that administration of Se in combination with DFMO protect cells from the harmful effect of high levels of polyamines.