Abstract: Starch hydrolyzing and α-amylase producing micro-fungi were isolated from soil. Among the isolated fungal species Aspergillus fumigatus and Chaetomium globosum were found prominent starch hydrolyzing and amylase producing fungi. Maximum starch hydrolysis was recorded in 6 to 7 days old culture for both A. fumigatus and C. globosum. However, α-amylase activity was found higher in 9 to 11 days old culture. Starch hydrolysis, α-amylase and protease activity of A. fumigatus was found higher as compare to C. globosum. At higher temperature (45 °C) also performance of A. fumigatus was better than C. globosum. As Aspergillus fumigatus was found to be better thermotolerant, it can therefore be utilize in starch processing industry as well as for amylase production at high temperature level.