Abstract: Background and Objective: Being significant zoonotic bacteria, Campylobacter spp. does not spread from broiler to human only via consumption of contaminated meat but also through the handling of live broilers and during the preparation of contaminated meat and meat products. This baseline survey was conducted to estimate the prevalence of Campylobacter spp. colonization and its associated risk factors in broilers of Mirsharai Upazila. Materials and Methods: From October to December 2019, pooled cloacal swab samples were collected from broiler farms. Standard bacteriological and molecular techniques were followed to isolate and identify Campylobacter spp. A standardized questionnaire was used to collect epidemiological data. Risk factors have been considered at the farm level. Results: Of 20 pooled cloacal swab samples from 20 broiler farms the prevalence of Campylobacter spp. was estimated to be 45% (95% CI-23.1-68.5). In risk factor analysis, the factors significantly associated with Campylobacter colonization were shed number, flock size, water supply, floor type, litter type, farmer’s experience and use of distinct cloth or separate footwear while entering the farms. Most of the farms had a 2.5-5% mortality rate and coccidiosis and necrotic enteritis were the most frequent causes of death. Farmers used a wide range of antibiotics mainly for growth promoters or prevention purposes rather than therapeutic purposes. Conclusion: The study gathered evidence of the presence of Campylobacter spp. colonization in broiler flocks and identified the factors that could aid in the development of effective strategies for managing Campylobacter colonization in chickens to prevent campylobacteriosis in humans via broilers.