Abstract: Background and Objective: Layer farming is one of the most important and vast growing agribusinesses due to the high global demands for eggs. Chicken eggs are the best source of quality protein, vitamins, minerals, fats and are badly needed by the many millions of people who live in poverty. Therefore the current study was designed to find out the effects of (Novizyme) enzyme supplementation on parameters of performance and egg quality of Hi-sex layer hens. Materials and Methods: The study was conducted at the poultry production unit, training and research farm, College of the Animal Production University of Bahri, Elkadaru Area, Khartoum North. Sixty commercial layer hens (hi-sex) breed at 53 weeks of age were used. Birds were kept in a semi-closed housing system equipped with battery cages. Layer hens were randomly assigned into four dietary treatment groups (0, 0.05, 0.075 and 0.1% of feed) in a complete randomized design. Results: The supplementation of Novizyme in different graded levels in layers diet showed no significant effects in all measured parameters of performance, internal and external egg quality characteristics throughout the experimental period. Conclusion: Based on the obtained result of the present study, It can be concluded that the inclusion of Novizyme enzyme on layers diet at the level of inclusion tested do not improve performance and egg quality characteristics.