Abstract: Background and Objective: Dynamic changes in testicular macro- and microstructures occur throughout sexual maturation. The present work was conducted to account for the temporal changes in the indigenous sheep testis during postnatal development. Materials and Methods: A total of twenty-one indigenous sheep, varying in ages from day 0-7 months, were divided into seven age groups (n = 3) at birth or day 0, 1 and 2 week, 1, 2.5, 5 and 7 month of postnatal age. Results: A rapid testicular development was observed after 2.5 months of age in indigenous lambs as determined by a significant (p<0.05) increase in testicular size. Centrally placed gonocytes migrated centrifugally towards the basement membrane with the progression of age and converted to spermatogonia and placed among the Sertoli cells at the periphery of the sex cords by the 2.5 months of age. Lumenization of seminiferous tubules and stratification of seminiferous epithelium, i.e., initiation of spermatogenesis in indigenous sheep were occurred at the 5th month of age. All types of the cell of spermatogenic lineage were found by 7 months of age. The presence of spermatozoa adhering to the adluminal border of the Sertoli cells as well as in the lumen of seminiferous tubules indicates the onset of puberty, i.e., the establishment of spermatogenesis, was to be established at 7 months of postnatal age in the indigenous sheep. Conclusion: Overall, this study represents the first analysis of the sequential changes in macro and microstructure and seminiferous epithelium of the indigenous sheep testis during postnatal development until puberty.