Abstract: Background and Objective: African animal trypanosomiasis is an economically important cattle disease in Sub-Saharan Africa and Cameroon in particular. This study aimed to determine the prevalence of bovine trypanosomiasis and risk factors in the Ngaoundere Abattoir. Materials and Methods: A cross-sectional survey was conducted for the first time from January to December 2017, at the Ngaoundere Municipal Abattoir (NMA) to fill this gap. A total of 739 blood samples from cattle slaughtered at the abattoir were collected and examined parasitologically using the buffy coat technique (BCT). Results: Study showed the presence of trypanosomiasis in slaughtered cattle with an overall parasitological prevalence of 12.72% and significantly higher during the rainy season (15.60%) than in the dry season (10.44%). Trypanosoma congolense (43.62%) was the predominant trypanosome species infecting the animals and closely followed by Trypanosoma brucei (36.17%), Trypanosoma vivax (12.76%) and mixed infections (7.45%). A very low parasitaemia (102-103 trypanosomes mL–1) was observed in 85% of the infected cattle. The mean packed cell volume (PCV) value of parasitaemic cattle (29.24%) was lower than that of aparasitaemic cattle (30.05%). Of the 182 cattle with poor body condition scores (BCS), 11% were infected. However, trypanosomiasis did not significantly affect PCV and BCS. Conclusion: Bovine trypanosomiasis occurs in cattle slaughtered in the NMA and the majority of these animals originate from the tsetse infested area of Mayo-Rey in North Cameroon.