Abstract: Background and Objective: Fasciolosis is an endemic disease that causes severe economic conditions and affecting cattle populations even the human. The control of snail population is major tool in reducing the incidences of fasciolosis. The present study was designed for studying the effect of dried root powder of Potentilla fulgens (P. fulgens) use as molluscicides against vector snail Lymnaea acuminata (L. acuminata). Materials and Methods: Toxicity experiment of different organic extracts and column purified of P. fulgens was continuously observed for 96 h at different concentration. Mortality was observed for 24, 48, 72 and 96 h. Six aquariums were setup for each concentration. The control group animals were kept in the equal volume of water under similar conditions without treatment. Mortality of snails was recorded at interval of 24 h each up to 96 h by using POLO computer programme. Results: The dried root powder of P. fulgens at 96 h LC50 against L. acuminata was 133.62 mg L–1. Among different organic extracts, ethanol extract was more toxic than other organic extract. The ethanol extract of P. fulgens was more toxic (24h LC50-108.65 mg L–1) against L. acuminata. The 96 h LC50 of column purified fraction of dried root powder of P. fulgens was 28.69 mg L–1. Conclusion: The present study showed that the product of P. fulgens has potent molluscicidal activity. The product of P. fulgens may be used as potent molluscicides.