Abstract: Objective: This study was conducted to evaluate the effect of feeding long chain fatty acid calcium salts (LCFA-Ca) on meat quality, blood parameters and nucleotide compounds of Hanwoo (Korean native cattle). Methodology: A total of 69 animals with mean body weight of 599±5 kg were divided into three treatments as control (n = 20), group 1 (n = 25) and group 2 (n = 24) were fed (1) control diet with no added fat, (2) 50% linoleic acid and 24% oleic acid containing LCFA-Ca and (3) 26% linoleic acid and 50% oleic acid containing LCFA-Ca, respectively 100 g day–1 animal–1 on Dry Matter Required (DMR) basis. Results: For meat quality, highest cooking loss, shear force and lowest pH was observed in group 1, compared with other two groups, but not reached significant level. The CIE a* value was significantly higher in control group (p<0.05), whereas no significant differences of L* and b* among the groups. Nucleic acid related compounds IMP, ADP and inosine tended (p<0.05) to be greater in group 2 compared to other two groups, whereas AMP was high in control. Group 1 had the highest MDA content than other groups (p<0.05). Fatty acid compositions including SAF, PUFA and ω6/ω3 were significantly higher in group 2 than the rest of 2 groups (p<0.05) but did not affect the percentage of MUFA. Serum concentration of triglycerides and calcium were greater (p<0.05) in Hanwoo steers fed LCFA-Ca than control group. Cholesterol, low-density lipoprotein (LDL) and high-density lipoprotein (HDL) concentrations were greater in group 2 without any significant effect. Conclusion: Therefore, it can be concluded that LCFA-Ca had impact on meat quality traits of Hanwoo including blood parameters and nucleotide compounds.