Abstract: Objectives: This study was performed to examine effect of varying level of protein and energy in pre-starter feed for 7 days old chicks on pectoralis muscle development of kampung super chicks. Materials and Methods: Fourthy kampung super day old chicks were fed pre-starter diet during the experimental period and were divided into four groups. The control group chicks were not fed for the first 3 days post-hatch and continued with a standard pre-starter diet to 7 days old. The second group was chicks with pre-starter type A diet with 20.19% of protein and 3300 kcal kg–1 of Metabolism Energy (ME) for the first day post-hatch to 7 days old. The 3rd group was treated with pre-starter diet type B for the first day post-hatch to 7 days old with protein level 21.84% and 3100 kcal kg–1 of ME and the last group was given pre-starter diet type C with with protein level 21.13% and 2800 kcal kg–1 of ME for the first day post-hatch to 7 days post-hatch. The parameters measured were body weight, pectoralis thoracicus weight, muscle area, cross-sectional area of myofibers and the number of Proliferating Cell Nuclear Antigen (PCNA)-positive nuclei. The data were analyzed using one way ANOVA, followed by Tukey tests. Results: The results showed that groups 4 had lower body weight, pectoralis thoracicus weight, muscle area, cross-sectional of myofibers area and number of PCNA-positive nuclei compared to group 2 and 3 (p<0.05) which proved that early post-hatch feed in kampung super chicks was important to body weight and pectoralis muscle development. Conclusion: It was concluded from the study that diet with 21.84% of protein and 3100 kcal kg–1 of ME in 7 days old chicks promised optimum performance and pectoralis muscle development for kampung super chicks.