Abstract: Tuberculosis caused by different species of genus Mycobacterium or different serotypes of various species are the leading cause of mortality among livestock, domesticated animals and humans alike. This leads to huge economic loss in terms of animal and human capital. Currently one third of global population is infected with tuberculosis (TB). There might be innumerable reasons for it being pandemic but proper diagnosis or lack of it is one of the major contributing factors for its global spread. In developing countries precise and reliable diagnosis has emerged out to be the major cause translating into high burden. The TB diagnosis has evolved over the time with changing needs from classical microscopic sputum smear analysis to rapid PCR based molecular diagnostics. Molecular techniques are becoming confirmatory diagnostic tools and advanced procedure for TB detection. Current review lays emphasis on the tuberculosis from lower animals to higher animals including human with respect to diagnostics, therapy and its improvisation over a decade.