Abstract: Objective: This experiment was conducted to assess the effect of a diet containing full-fat canola seed and supplemented with rosemary leaves and vitamin C on broiler performance, carcass characteristics, meat fatty acid (FA) and lipid oxidation of broiler meat. Methodology: Two separate groups of 1 day old (d1) male broilers were fed from day 15-40 a diet containing 5% of canola seed and 27% of soybean meal as a control group. From days 40-56 chicks were allocated to three homogeneous groups. One group was fed a control diet without antioxidant, while the other two groups were fed with (canola seed supplemented with 10 g kg–1 of rosemary leaves) or 200 mg kg–1 of vitamin C. Birds were slaughtered at 56 days of age. After evisceration, thigh meat samples were separated, frozen at -20°C until to determine the fatty acid profile or stored at 4°C in the dark until to determine the lipid oxidation. Results: Results showed that the diet containing 5% of canola seed reduced (p<0.05) chicken growth by almost 4% at 35 and 40 days compared to the control diet. However, performance parameters of chicks were generally improved by the addition of rosemary leaves and vitamin C. The inclusion of canola seed increased (p<0.05) the concentration of omega-3 FA (2.14 and 1.79 against 0.85%) in meat, especially the proportion of α-linolenic acid (1.53 and 1.24 against 0.66%) and the polyunsaturated fatty acids: saturated fatty acids ratio and decreased widely the n-6: n-3 ratio. Dietary rosemary leaves were more effective (p<0.05) in inhibiting lipid oxidation of the thigh meat compared to vitamin C during storage at 4°C. Conclusion: These results indicate that the simultaneous use of rosemary and ground canola seed improves broilers performance and meat quality.