Abstract: This study describes the ultrasonographic findings of the urinary tract of buffalo calves suffered from urine retention. In this study, 25 male buffalo calves with urine retention and 8 apparently clinically healthy male buffalo calves were used. All calves were subjected to thorough clinical and ultrasonographic examinations. Based on the clinical findings, the diseased calves were classified into four groups. The first group included 8 calves with severe abdominal pain and absence of urine outflow, second group included 5 calves with mild abdominal pain and absence of urine outflow, third one included 8 calves with absence of both pain reactions and urine outflow and finally, fourth group included 4 calves with severe abdominal pain and dribbling of bloody urine. Both transcutaneous and transrectal ultrasonographic examinations were carried out by using 3.5-5.0 MHz convex and 6-8 MHz linear transducers. Ultrasonographic examination revealed distended urinary bladder in first, second and fourth groups. In third group, the urinary bladder was ruptured with anechoic to hypoechoic fluid accumulated in the abdomen. In the second group, the anechoic urine was present inside and outside the urinary bladder. Hydronephrosis of variable degrees was detected in all diseased calves. Moreover, urethral calculi could be detected in 4 calves at 3rd group. Renal calculi were seen in a calf at 4th group. Both renal and urethral calculi appeared as hyperechogenic dots with acoustic shadowing. In conclusion, ultrasonography may be considered a diagnostic and prognostic tool for urine retention in buffalo calves.