Abstract: Objective: The effect of breed, apoVLDL-II gene polymorphism and metabolic biochemical markers on growth and body composition traits were investigated in 400 different four chicken breeds (Cobb, Hubbard, Aves and Ross). Methodology: Blood samples were collected from chickens of each breed for DNA extraction and biochemical assay. Association analysis of the impact of breed revealed that Ross breed has the highest measurements for growth and body composition traits except for feed intake; where highest feed intake was recorded for Aves one. Results: The PCR-BfmI digestion of 492 bp of a fragment of intron-I of apoVLDL-II gene revealed two fragments (396 and 96 bp) for genotype BB, three fragments (492, 396 and 96 bp) for genotype AB and undigested fragment (492 bp) for genotype AA. Statistical analysis indicated that there was significant association between apoVLDL-II genotypes and growth and body composition traits. The AB genotype was higher than both BB and AA genotypes in all growth and body composition traits except for feed intake. In concern to metabolic biochemical data, broiler breeds could be significantly (p<0.05) affect serum levels of T3 and T4 to reach the peak value in Ross and minimal value in Aves breed. These data could be compatible with growth and body composition data. Meanwhile, the level of plasma proteins or lipid profile do not showed a significant different between breeds. Conclusion: This study highlights the possibility of breed effect, apoVLDL-II/BfmI locus and metabolic biochemical markers as candidates for growth and body composition traits in chickens.