Abstract: The study describes the effect of stabilization on the stability of an indigenous live attenuated orf vaccine with stabilizers like LS, LHT and TAA after lyophilization and also reconstitution with NSS, PBS, distilled water and 1 M MgSO4 exposed at different temperatures under established lyophilization conditions. The stability of the vaccine was assessed both in freeze-dried and reconstituted forms at different temperatures. The results indicated that the orf vaccine lyophilized with LS stabilizer at 25 and 45°C and LHT at 37°C found superior over others in stabilizing the keeping quality of the vaccine. Intrinsic thermo-stability studies revealed the rapid deterioration of the vaccine, when compared to the vaccine stabilized with the either of the stabilizers. Among the diluents used for reconstitution of the vaccine, quality in terms of infectivity titers of the virus was well preserved in vaccine diluted with 0.85% NSS compared to other diluents like PBS, DW and 1 M MgSO4. The 1 M MgSO4 found unsuitable for diluting the orf vaccine. The study suggests that the LS at 25 and 45°C and LHT at 37°C are the choice of stabilizers and 0.85% NSS is the choice of diluent for orf vaccine at all temperatures contemplated.