Abstract: The aim of this study is to determine the influence of physiological status on blood metabolic profile, enzymes and some hormones concentration in the blood of Egyptian buffalo. Investigations were carried out on 24 buffalo (12 pregnant buffalo cows on the 60th day prior to parturition and 12 lactating buffalo cow from 10th day of lactation during winter feeding season). The buffalo cow was average 3 years old and healthy. Buffalo were fed according to Mehlet Moussa Experimental Station which belongs to Animal Production Research Institute, north part of the Nile Delta, Egypt. The present study indicated that there was drop in calcium, sodium, phosphorus and potassium levels during early stage of lactation than pregnant period buffaloes, the opposite trend recorded in chloride levels. The concentrations of plasma glucose, urea, cholesterol, triglycerides and total protein were higher during pregnant period than in lactation period, the opposite trend recorded in creatinine level. The present study indicated that, the blood enzymes (AST, ALT and AlP) activities were insignificantly higher during pregnant period than in lactation period. The concentrations of plasma IGF-1, thyroid hormones and leptin were higher during pregnant period than in lactation period.