Abstract: Typhlectomy has been prescribed for the treatment of cecal affections in dogs. The effect of suture materials on healing process after typhlectomy has not been studied yet. Twenty seven Mongrel dogs were randomly divided into 3 groups. Chromic catgut, silk and vicryl were used for groups 1, 2 and 3, respectively and 3 dogs each were euthanized on the 7th, 14th and 21st POD for evaluation of the healing process of colonic wounds. None of the animals had post-operative abnormal clinical signs. Non-significant differences in the mean height of the ridges were observed among the 3 groups. Groups 1 and 2 showed a moderate to severe mucosal necrosis at free part of inverted colonic edges, minimal cellular infiltration in lamina properia, inflammatory cell infiltration in tunica muscularis and moderate to severe tissue reaction around the suture material whereas, group 3 exhibited least mucosa necrosis, minimal destruction of tunica muscularis and low inflammatory cell reaction around suture material. Scores of these lesions did not differed significantly among the 3 groups but tissue reaction around the suture material differed significantly (p<0.05). In conclusion, vicryl was the most convenient and superior suture material for colonic wound healing after typhlectomy in dogs.