Abstract: The prevalence of clinical endometritis in dairy cows was surveyed in Northeast China between March 2012 and August 2012. A total of 1370 apparently healthy dairy cows were detected from three provinces (Heilongjiang, Jilin and Liaoning) that locate in Northeast China. All uterine swabs and samples of vaginal mucus were collected and all of them were microbiologically analyzed. The results showed that the overall prevalence of clinical endometritis in dairy cows was 17.4% and the prevalence is similar in three different province which Heilongjiang (16.7%, 90/540), Jilin (17.6%, 74/420) and Liaoning (18.3%, 75/410). The prevalence in intensively reared dairy cows (11.9%, 107/900) was lower than that in semi-intensively reared dairy cows (28.1%, 132/470) (p<0.05). The dominant bacterial isolates in the study were Staphylococcus aureus (21.8%), Streptococcus sp. (19.2%) and Arcanobacterium pyogenes (15.0%). The results of this survey indicated the presence of clinical endometritis in dairy cows in Northeast China and rearing system is an important factor to affect the clinical endometritis in dairy cows in Northeast China and Staphylococcus species was major pathogen.