Abstract: In neonatal buffaloes calves Cryptosporidium spp. infection was investigated to determine its importance as etiological agent of diarrhoea syndrome. A total of 162 faecal samples were collected from neonatal buffalo calves of below 5 months age and examined for the Cryptosporidium spp. infection. Overall prevalence of the disease was observed as 38.3%. A gradual decline in the prevalence values was seen with increase in the age, highest in 0-30 days age group i.e., 65.71% and lowest in 4-5 months age group i.e., 5.88%. This trend of decline in prevalence values was observed in both diarrhoeic as well as non-diarrhoeic calves. A high degree of association was seen between Cryptosporidium infection and diarrhoea with infected cases at relatively higher risk to diarrhoea than non-infected. The highest prevalence (40.65%) of infection of was recorded during the monsoon season followed by pre monsoon season (39.35%) and lowest prevalence (34.04%) was recorded in the post monsoon season. Female calves showed higher prevalence (40.35%) than the male calves (33.3%).