Abstract: The study was conducted from November 2008 to May 2009 to assess the antimicrobial sensitivity pattern of 22 Salmonella isolates viz., 15 Salmonella Gallinarum strains recovered from various sources of different regions of India including 3 reference strains and 5 other Salmonella enterica serovars (7 isolates) by testing with 16 different antimicrobials. All the isolates including the standard reference Salmonella strains were subjected to antimicrobial sensitivity test using 16 different antimicrobial agents by disc diffusion method. All the Salmonella Gallinarum isolates showed resistance to erythromycin and 86.7% of them were resistant to nalidixic acid. More than 53% of the Gallinarum isolates were either 100% resistant or less sensitive to the commonly used antimicrobials, kanamycin and tetracycline whereas about 93.3% of them were sensitive to gentamicin and amoxicillin/clavulanic acid. Antimicrobial sensitivity pattern for ciprofloxacin, ofloxacin, colistin and sulfa-trimethoprim was around 88.8 and 82% of the isolates were sensitive to enrofloxacin and chloramphenicol. Among other serotypes included in the study S. typhimurium showed maximum resistance against 6 antimicrobials followed by S. kastrup which was resistant to 5 antimicrobials. S. typhimurium was 100% sensitive only to ciprofloxacin. In overall, out of a total of 22 isolates tested for different antimicrobials 4/22 (18.2%) were resistant to at least one antimicrobial and the remaining 81.8% were resistant to at least two or more antimicrobials, supporting the fact for the emergence and widespread presence of multidrug resistant Salmonella species and the importance of the implementation of suitable measures to avoid indiscriminate use of antibiotics in food animals.