Abstract: The aim of the present study was to determine how the effect of adding different amounts of canola meal, as a well known protein supplement in animal nutrition, on growth parameters and performance of male Wistar rats. Daily Weight Gain (DWG), Daily Feed Intake (DFI) and Feed Conversion Ratio (FCR) were registered 48 male Wistar rats were divided into four groups of 12, each in 2 separate cages of 6. Three experimental groups were fed diets containing 10, 20 and 30% canola meal (E10, E20 and E30, respectively) and the fourth one (control) was with no canola meal added. In a 6 weeks period trial, adding canola meal did not change ADWG but reduced ADFI. Partial substitution of soybean meal (SBM) by Canola Meal (CM) caused an increase in FCR. Results showed that independently of percentage of SBM substitution, adding canola meal, especially in E20 diet, slightly improved the animal performance and therefore can be used in laboratory animals feeding.