Abstract: The prevalence of antibodies to Neospora caninum and Toxoplasma gondii were investigated in seven lions (Panthera leo) from Hann’s zoo of Dakar-Senegal. Seven sera samples were examined for antibodies against Neospora caninum (Neospora caninum antibodies test kit, cELISA) and Toxoplasma gondii (ID Screen® Toxoplasmosis Indirect ELISA). All sera were positives to Neospora caninum antibodies whereas 3 for 7 (42.86%) were positives to Toxoplasma gondii. Serological results indicate a common exposure to Neospora caninum and Toxoplasma gondii among lions (Panthera leo) from zoo in Senegal.