Abstract: There is a direct relationship between the production of honey bee and population of colony. Using long lasting comb may be offset by deleterious effects of old comb acting as a biological constraint on larval development. In this research compared the quantity of brood produced, average body weight of adult bee and population of adult bees in hive on brood combs of either old and new bee wax. Old combs were of an unknown age, were dark and heavy as typical of combs one or more years old. We placed the old combs in to strong colonies to clean them of debris. In this study accomplishment between times tow year on days 14 and 21. In the development of brood or population season measured for each colony the area (cm2) of all brood including eggs, Larvae and brood. T-test used for data analysis. There were significant differences between new and comb waxes in terms of brood population in years 2005 and 2006 (p<0.01). Also the significant difference has been found for honey production and weight of honey bees between old and new combs wax at years 2005 and 2006. It is concluded that, new combs are effective on brood development and honey production. Thus, could be suggesting that beekeepers should eliminate old combs from their operations.