Abstract: This study was conducted to determine the effect of replacing different levels of rapeseed meal with soybean meal on the performance of commercial laying hens. Four levels of rapeseed meal; 0, 5, 10 and 15% were used in diets of 144 Hi-Line (W-36, Strain) laying hens from the age of 44 to 56 weeks. Hens were distributed in a randomized complete block design with 4 treatment, 3 replicates and 12 hens in each replicate. The parameters measured were the feed intake, hen-day egg production, egg mass, feed conversion ratio and egg weight. With increasing of rapeseed meal level in diets, feed intake was showed elevation (p<0.05). Egg weight was significantly higher when birds fed 10% rapeseed meal (p<0.05). No specific trend was observed on the effect of rapeseed meal on egg mass and feed conversion ratio, however these parameters were higher in groups that fed 10% rapeseed meal. With increasing rapeseed meal in diets, production ratio was decreased. It was concluded that; the replacement of 10% rapeseed meal with soybean meal in the layer diets might be useful and had economical benefits for producers.