Abstract: The aim of this study was to examine the effectiveness of intra-peritoneal administration of honey in prevention or reduction of post-surgical peritoneal adhesions in dog. The study was conducted with 18 adult, female, mixed breed dogs with mean weight of 20±4.25 kg. A standardized surgical trauma (serosal trauma model) was made in the dogs’ descending colon to induce adhesion formation. Control group (n = 9) received saline treatment and in experiment group (n = 9) the lesions were covered with honey. Three weeks after laparotomy, the dogs were sacrificed and the adhesions were graded according to their degree of severity based on Evans scoring system. Overall, 66.66% (n = 6) of the control animals and 22.22% (n = 2) of the experiment animals presented with moderate (grade 2) and severe (grade 3) adhesions. In the control group, there were 3 (33.33%) dogs with grade-0 or grade-1 adhesions but in the experiment group there were 7 (77.77%) dogs with the same grades of adhesions. The difference between two groups was shown to be significant (p<0.05). Based on the results of this study, it was concluded that honey appears to be a useful covering for damaged peritoneal surfaces and had high effectiveness in reducing post-operative adhesions.