Abstract: The aim of this study was to describe glucose, GOT, γ-GT, B-OH, GSH-PX, calcium and phosphorus concentrations in healthy Alpacas population under farming conditions. Thirty-six clinically healthy female Alpacas (Lama pacos) were housed in three different Chile regions (North Zone; Central Zone; South Central Zone) at three different altitudes. Blood samples were collected 4 times (Spring, Summer, Autumn, Winter) for one year period. Two-way repeated measures analysis of variance (ANOVA) were used to determine significant differences and p<0.05 were considered statistically significant. Bonferroni’s test was applied for post hoc comparison.