Abstract: Study was conducted in parts of northern India having tropical climate with two prominent type of rearing and management system for cattle. Cattle that are reared and managed by farmers (group A) who rarely supplement mineral mixture in the ration and cattle of organized farms that are managed on good quality ration and supplemented with mineral mixture (group B). Study revealed significant (p<0.05) difference in mineral concentration in cattle with respect to copper, zinc, iodine, cobalt, calcium and phosphorus between group A and group B in various districts. Iron was in adequate concentration in both groups. The concentration of serum retinol and α-tocopherol was lower in group A cattle. The concentration of serum ALT and AST in cattle of both group A and B were towards lower side but no significant difference (p>0.05) was found between groups. SAP, Cp was significantly (p<0.05) different between the groups in cattle of all the four districts. The concentration of both T3 and T4 in cattle of group A was lower than healthy cattle. However serum T4 concentration was better indicator of such deficiencies. Status of serum retinol, α-tocopherol, B12, SAP, Cp, T3 and T4 can be used as markers to evaluate status of serum minerals. Mineral supplementation results in better status of minerals in cattle but commercial available mixtures are not appropriate to fulfill the requirement of these cattle.